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Circular Dichroism, often abbreviated to "CD", is displayed when an optically active substance absorbs left or right handed circularly polarized light preferentially. The difference in left and right handed absorbance A(l)- A(r) is often very small hence the J-810 provides a CD resolution of 0.0005 millidegrees when set to read at ±10 millidegrees. CD signal varies with wavelength and our current model J-815 CD spectrometer is capable of measuring over a wavelength range of 900 nm to 163 nm. CD spectra for peptides, proteins and nucleic acids differ depending upon their secondary structure and the analysis of CD spectra provides valuable information about the secondary structure of biological macromolecules. |
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