FP-6500 - High Performance Research Fluorescence Spectrophotometer

Research Fluorescence Spectrophotometer

 

 

FP-6500 Research Fluorescence Spectrometer

research fluorescence spectrophotometer 

JASCO’s latest research grade Fluorescence Spectrophotometer combines excellent specifications and a flexible optical system with JASCO's unique cross platform spectroscopy software suite Spectra Manager™.
The FP-6500 was designed to meet the demanding needs of laboratories performing biochemistry applications like kinetics, stopped-flow and rapid kinetics,  titration and fluorescence anisotropy experiments. These applications can be easily and rapidly undertaken using dedicated accessories and software.

Many advanced features of the FP-6500 are supplied as standard with the instrument; including spectral correction using Rhodamine B as well as phosphorescence spectrum measurements and phosphorescence lifetime determinations. If extended wavelength measurements are required a NIR instrument, the FP-6600 is available which extends wavelength measurements to 1010 nm.

Excellent optics and high sensitivity

FP-6500/6600 optical system utilizes high quality components. The source is a DC-powered 150W Xenon lamp (in a sealed housing) which employs a photometric ratio system. This utilizes a second photomultiplier tube to monitor and compensate for any variations in the intensity of the 150 Watt Xenon source thus ensuring maximum analytical stability. The use of concave holographic grating monochromators with optimized blaze angles provides maximum sensitivity over the entire wavelength range. Rapid slew rates of 30,000 nm/min and wavelength scan rates from 10 nm/min to 20,000 nm/min for both excitation and emission monochromators gives the speed and flexibility necessary when performing 3-D or multiple scanning applications. 

High quality optical system

The optical system utilizes high quality components including a DC-powered 150W Xenon lamp (in a sealed housing), holographic concave grating monochromators, fast EX and EM direct scanning drive, variable slits and horizontal sample illumination. Especially, minimum spectral bandwidth of 1 nm on both EX and Em monochromators provides high resolution data. 

High sensitivity

The FP-6500 boasts extremely high sensitivity with a signal to noise ratio greater than 800:1 using the Raman band of water, peak-to peak, SBW 10 nm EX and 10 nm EM, using a 2 second response. Or 3200:1 RMS under the same conditions.

Versatility and flexibility for biochemistry

The FP-6500 is supplied with a single position water thermostattable cell holder. In addition, a variety of accessories for temperature control and specific application areas are available including a stirred peltier thermostatted single cell holder, 8-position automatic water thermostat turret cell changer, peltier thermostat cell holder with automatic polarizer / depolarizer, sample holder lid with syringe port for fluorescence and chemiluminescence experiments, automatic titration accessory for evaluating the denaturation of proteins or ligand binding experiments as well as stopped flow accessories. These accessories can be used in conjunction with application specific software for a total solution to your application requirements. Fast data collection rates are available and can be optionally increased to provide data collection rates of up to 0.1 milliseconds essential for stopped flow and fast kinetics applications. Spectral bandpass is selectable from 20 nm to 1 nm for when high resolution is required with additional reduced height slits of 5 nm and 10 nm on both monochromators for when only small sample volumes are available and micro cells are used. 

GLP/GMP compliance

The FP-6500 was designed to meet requirements of GLP/GMP compliance and is supplied with JASCO's validation software. The FP-6500 validation program is provides the following tests: wavelength accuracy, wavelength repeatability, resolution, stray light, detection limit and photometric stability. UV-30 filter, L-39 filter, R-60 filter and secondary low pressure mercury lamp are built in the FP-6500/6600. Rhodamine B, diffuser plate, attenuator, reflection plate and triangle cell are standard.

Compact design

The compact optical bench is housed in a rugged case measuring only 550(W) X 540(D) X 300(H) mm.

Optional Accessories for Bio-Applications

6808-J021A

FMH-110 Micro cell holder

6808-J022A

FMM-100 Micro quartz cell for FMH-110

6808-J013A

STR-313 Water thermostattable cell holder with stirrer

6808-J001A

ETC-273 Peltier thermostatted single cell holder with stirrer (Water-cooled)

6808-J002A 

EHC-573 Peltier thermostatted single cell holder with stirrer (Air-cooled)

6808-J008A

 FCT-133 Water thermostattable automatic 8-position turret cell changer

6808-J006A 

CSP-143 Sample holder lid with syringe port

6808-J003A

ADP-303 Peltier thermostatted depolarization accessory (Water-cooled)

6808-J004A

APH-103 Peltier thermostatted depolarization accessory (Air-cooled)

6808-J026A

ATS-443 Automatic titration accessory

Optional Accessories for Environmental or Routine Applications

6808-J010A 

QFS-253 Vacuum sipper

6808-J009A

SHP-393 Peristaltic sipper

6808-J024A

FHM-400 High sensitivity cell holder

6808-J016A

FSC-423 Micro flow cell unit with 15µl cell

6808-J017A

FSC-523 Micro flow cell unit with 30µl cell

6808-J018A

FSC-623 Micro flow cell unit with 100µl cell

6808-J011A

MFC-233 HPLC flow cell accessory

6774-J001A

ASU-605 Autosampler

Other Optional Accessories

6808-J025A

FSA-400 30° incident angle cell holder

6808-J023A

FDA-430 Solid sample holder

6808-J012A

 EFA-383 Epifluorescence accessory

6808-J020A

 FUV-420 Accessory for UV/Visible measurement

6808-J019A

 FDP-203 Polarizer and analyzer accessory (for UV/Visible range)

6808-J029A

 FDP-213 Polarizer and analyzer accessory (for Visible range)

6808-J014A

PMA-283 Low temperature measurement accessory

6808-J027A

WRE-343 PM tube for extended wavelength range

6808-J007A

ESC-333 reference light source

6808-J028A

ROU-213 Analog output I/F (factory option)